罗仁治疗儿童肾病综合征蛋白尿经验介绍＊

本文介绍了罗仁教授治疗儿童肾病综合征蛋白尿的学术思想及理法方药。肾病综合征蛋白尿属中医学的“水肿”“尿浊”“虚劳”“腰痛”范畴，发病主要与肺、脾、肾三脏功能失调有关。蛋白尿是肾病综合征的必要诊断条件，不仅可直接导致低蛋白血症，引发水肿，还会促进肾功能的恶化。罗仁教授认为尿蛋白源于血浆，是水谷精微，为至阴之精，应固藏于肾，并且有赖于肺之宣发、脾之升清、肝之疏泄的共同作用，创立了肾病1号方从肺、脾、肝、肾四方面减少尿蛋白，使患者更早进入缓解期，延缓疾病进展，调节免疫，防止复发，尤其对激素抵抗、激素依赖患者具有更重要的意义，并随证或随症加减，对于病情复杂的复合病或复合证患者可用单双日疗法调整阴阳，单日用肾病1号方减少蛋白尿，双日用小生六汤调和脏腑，还从心理、运动、饮食三方面指导患者积极面对疾病。     

染料木黄酮对前列腺癌细胞增殖、迁移和侵袭的影响及机制

本研究拟探讨染料木黄酮对前列腺癌细胞LNCaP和CWR22RV1的增殖、迁移和侵袭能力的影响。一、材料与方法1.材料:前列腺癌细胞株LNCaP和CWR22RV1(中国科学院上海生命科学研究院);Genistein(天津科瑞泰科技有限公司);RPMI 1640、胎牛血清(FBS)、胰蛋白酶(美国Gibco公司);E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin,Abcam公司);聚氰基丙烯酸正丁酯(BCA)试剂盒、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)上样缓冲液(×5)及蛋白质印迹法(Western blot)试剂盒(上海市碧云天生物公司);2.实验方法:采用噻唑蓝(MTT)实验、划痕实验和Transwell实验检测Genistein对细胞增殖、迁移及侵袭能力的影响。采用Western blot检测E-cadherin、N-cadherin、Vimentin、CD44和Oct-4的表达水平。     

品管圈对缩短结直肠癌患者术后胃肠功能恢复时间的效果观察

目的探讨品管圈对结直肠癌患者术后胃肠功能恢复时间的临床效果。方法选取2019年4月至5月间上海市健康医学院附属嘉定区中心医院收治的52例结直肠癌术后患者为改进前组,2019年6月至7月52例患者为改进后组。检查改进前组患者平均排气时间、排便时间及腹胀发生率,采用品管圈对改进后组患者进行干预,比较两组患者胃肠道功能和相关无形成果。结果改进后组患者排气时间、排便时间和腹胀发生率均低于改进前组,差异均有统计学意义(均P<0.05)。通过品管圈活动,圈员们责任心、自我价值、团队精神、科研能力、沟通能力和解决问题能力均提升。结论品管圈可缩短结直肠癌患者术后排气时间和排便时间,降低腹胀发生率,提高圈员综合素质,值得推广。     

Association of Multiple Plasma Biomarker Concentrations with Progression of Prevalent Diabetic Kidney Disease: Findings from the Chronic Renal Insufficiency Cohort (CRIC) Study

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Identification of key genes and pathways in discoid lupus skin via bioinformatics analysis

Discoid lupus erythematosus (DLE) is the most common skin manifestation of lupus; however, the molecular mechanisms underlying DLE remain unknown. Therefore, we aimed to identify key differentially expressed genes (DEGs) in discoid lupus skin and investigate their potential pathways.To identify candidate genes involved in the occurrence and development of the disease, we downloaded the microarray datasets {"type":"entrez-geo","attrs":{"text":"GSE52471","term_id":"52471"}}GSE52471 and {"type":"entrez-geo","attrs":{"text":"GSE72535","term_id":"72535"}}GSE72535 from the Gene Expression Database (GEO). DEGs between discoid lupus skin and normal controls were selected using the GEO2R tool and Venn diagram software (http://bioinformatics.psb.ugent.be/webtools/Venn/). The Database for Annotation, Visualization, and Integrated Discovery (DAVID), Enrichr, and Cytoscape ClueGo were used to analyze the Kyoto Encyclopedia of Gene and Genome pathways and gene ontology. Protein-protein interactions (PPIs) of these DEGs were further assessed using the Search Tool for the Retrieval Interacting Genes version 10.0.Seventy three DEGs were co-expressed in both datasets. DEGs were predominantly upregulated in receptor signaling pathways of the immune response. In the PPI network, 69 upregulated genes were selected. Furthermore, 4 genes (CXCL10, ISG15, IFIH1, and IRF7) were found to be significantly upregulated in the RIG-I-like receptor signaling pathway, from analysis of Enrichr and Cytoscape ClueGo.The results of this study may provide new insights into the potential molecular mechanisms of DLE. However, further experimentation is required to confirm these findings.     

The effectiveness of specialized nursing interventions for patients with Parkinson disease: A randomized controlled study protocol

Background:The purpose of this experiment is to evaluate the impact of the care of Parkinson disease nurse specialist on improving motor symptoms and life quality for patients with Parkinson disease (PD).Method:This is a randomized controlled research, and it will be conducted from April 2021 to October 2021 at Sichuan Provincial People''s Hospital. The experiment was granted through the Research Ethics Committee of Sichuan Provincial People''s Hospital (004510293). All the patients suffer from PD, age 18 years or older, both female and male, regardless of the duration or severity of this disease are eligible. The exclusion criteria contains: lack sufficient knowledge to complete questionnaires, serious physical comorbidities or refuse to take part in the program. In our experiment, the major result measures are motor symptoms and life quality. For the measurement of life quality, we will utilize Parkinson disease Questionnaire-39, the most extensively utilized the scale of life quality in PD. The evaluation of motor symptoms severity is carried out with the revision of Unified Parkinson Disease Rating Scale sponsored by Movement Disorder Society.Results:Table 1 indicates clinical outcomes at different time points.Conclusion:The Parkinson''s disease nurse specialist care may promote the life quality in the PD patients.Trial registration number:researchregistry 6284.